Atanas Radkov

Scientist/Lab manager @ Arcadia Science

I'm a protein biochemist and have experience with over ten different classes of enzymes and proteins. I have developed many enzyme activity assays and solved several x-ray protein crystal structures. I am excited to pass my knowledge by mentoring students and other researchers.

Berkeley, CA 0000-0001-7418-279X atanas_radkov

All Pubs (2)Attributed Pubs (2)

A workflow to isolate phage DNA and identify nucleosides by HPLC and mass spectrometry

Adair Borges, Atanas Radkov, and Peter S. Thuy-Boun

Published: Dec 19, 2022

This pub details a process for phage amplification and concentration, DNA extraction, and HPLC and MS analysis of phage nucleosides. We optimized the approach with model phages known to use non-canonical nucleosides in their DNA, but plan to apply it for other phages.

Connections

Supplements (3): Protocol collection - Phage DNA isolation and chemical analysis - protocols.io • Mass spec data - Zenodo • Code v1.0 - GitHub

Inducing protoplast formation in Phaeodactylum tricornutum by silica deprivation, enzymatic treatment, or cytoskeletal inhibition

Prachee Avasthi, Cameron Dale MacQuarrie, and Atanas Radkov

Published: Mar 29, 2023

Treating P. tricornutum cells with serine endopeptidases or certain cytoskeletal inhibitors induces the formation of cell wall-free protoplasts and suggests a novel role for actin and myosin in preventing protoplast formation.