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Galo Garcia III
Scientist
Galo is a cell biologist. He earned a Ph.D from UC Berkeley in Molecular and Cell Biology. His favorite emerging research organisms are protists, because they harbor biological innovations that enable life in unexpected locations.
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Published: Aug 23, 2022
Cells can be highly motile, moving in and out of a microscope’s field of view. Understanding complex life cycles is difficult without continuous observation. To overcome this challenge, we’ve developed a 3D-printed microchamber device to confine cells for long-term visualization.
Connections
Supplements (7): 3D-printing files - NIH 3D Print Exchange • Code v1.1 - GitHub • Imaging data - C. steinii cyst rotation - Zenodo • Imaging data - C. steinii early encystment - Zenodo • Imaging data - C. steinii in a 384-well plate or microwells - Zenodo • Imaging data - C. steinii reanimation experiment - Zenodo • Imaging data - T. rostrata timelapse - Zenodo
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Published: May 03, 2023
Constraining motile microorganisms for live imaging often requires costly microfluidics or optical traps to keep them in view. We used patterned stamps and agar to make versatile, inexpensive “microchambers” and offer a way to predict the right chamber size for a given organism.
Connections
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Published: Jun 23, 2023
We’re crossing C. reinhardtii and C. smithii algae for high-throughput genotype-phenotype mapping. In preparation, we’re comparing the parents to uncover unique species-specific phenotypes.
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Published: Nov 17, 2023
We distilled label-free microscopy data by comparing and implementing feature-detection algorithms. Sobel and Laplacian methods outperformed pixel intensity variance in accuracy.
Connections
Supplements (1): Code v1.0 - GitHub
