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Created Apr 12, 2022

Inducing protoplast formation in Phaeodactylum tricornutum by silica deprivation, enzymatic treatment, or cytoskeletal inhibition

Prachee Avasthi, Cameron Dale MacQuarrie, and Atanas Radkov

Published: Mar 29, 2023

Treating P. tricornutum cells with serine endopeptidases or certain cytoskeletal inhibitors induces the formation of cell wall-free protoplasts and suggests a novel role for actin and myosin in preventing protoplast formation.

Distinct spatiotemporal movement properties reveal sub-modalities in crawling cell types

Prachee Avasthi and Ryan York

Published: Oct 14, 2022

Quantifying movement is a powerful window into cellular functions. However, cells can generate movement through a variety of complex mechanisms. Here, we generate a flexible framework for comparing an especially variable type of motility: cellular crawling.

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Supplements (1): Code v1.1 - GitHub

Chlorarachniophytes form light- and Arp2/3 complex-dependent extensions that are involved in motility and predation

Prachee Avasthi and Cameron Dale MacQuarrie

Published: Dec 04, 2022

Long protrusions from several microalgal species appear to help cells move, capture prey, transport mitochondria and chloroplasts, and more. Are they filopodia that evolved abilities more like other actin- or microtubule-based structures, or are they something new?